Intracellular free calcium levels are reduced in mitotic Pt K2 epithelial cells.

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Transition from metaphase to anaphase is accompanied by local changes in cytoplasmic free calcium in Pt K2 kidney epithelial cells.

We have used a Ca2+-sensitive dye, fura-2, to investigate the role of Ca2+ during mitosis in Pt K2 epithelial cells. The concentration of cytoplasmic free calcium, [Ca2+]i, increased 2-fold between metaphase and anaphase. Digital image analysis revealed two patterns of [Ca2+]i localization during anaphase. In half of the anaphase cells, the increase in [Ca2+]i was greatest in the region near th...

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 1985

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.82.3.800